Human Aspartate Aminotransferase ELISA kit (E01T0034) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Aspartate Aminotransferase ELISA kit (E01T0034)

E01A0626 Human Aspartate Aminotransferase ELISA kit

The Human Aspartate Aminotransferase ELISA kit can be used to identify samples from the human species. Aspartate Aminotransferase can also be called AST, Aspartate transaminase, aspartate aminotransferase, AspAT/ASAT/AAT, (serum) glutamic oxaloacetic transaminase (GOT, SGOT).

Products

Specifications of Human Aspartate Aminotransferase ELISA kit

Product Information
Cat. No.	E01A0626
Product Name	Human Aspartate Aminotransferase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 ng/ml
Sensitivity	1.0 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	50 ng/mL	1 vial
STANDARD C (0.5mL)	100 ng/mL	1 vial
STANDARD D (0.5mL)	250 ng/mL	1 vial
STANDARD E (0.5mL)	500 ng/mL	1 vial
STANDARD F (0.5mL)	1000 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

AST ELISA kit uses an anti-AST antibody and an AST-HRP conjugate in a competitive enzyme immunoassay method. AST-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AST antibody binding site between AST from samples and AST-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AST. Since the number of sites is limited, as more sites are occupied by AST from the sample, fewer sites are left to bind AST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AST concentration in each sample is interpolated from this standard curve.

Quality Control on Human Aspartate Aminotransferase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between AST and analogues was observed.