Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit (E01P0001)

BlueGene Biotech Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit (E01P0001)

E01P0001 Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

The Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit can be used to identify samples from the human species. ATP-Binding Cassette, Sub-Family B, Member 1 can also be called ABCB1, ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, ATP binding cassette subfamily B member 1, P-glycoprotein, P-glycoprotein 1, permeability glycoprotein, P-gp, Pgp, multidrug resistance protein 1, MDR1, ATP-binding cassette sub-family B member 1, ABCB1, cluster of differentiation 243.

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Specifications of Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Product Information
Cat. No.	E01P0001
Product Name	Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 ng/ml
Sensitivity	1.0 ng/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	50 ng/mL	1 vial
STANDARD C (0.5mL)	100 ng/mL	1 vial
STANDARD D (0.5mL)	250 ng/mL	1 vial
STANDARD E (0.5mL)	500 ng/mL	1 vial
STANDARD F (0.5mL)	1000 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ABCB1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ABCB1. Standards or samples are then added to the microtiter plate wells and ABCB1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ABCB1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ABCB1 are added to each well to “sandwich” the ABCB1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ABCB1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCB1 concentration in each sample is interpolated from this standard curve.

Quality Control on Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ABCB1 and analogues was observed.

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Summary of the Assay Procedure for Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

Citations of Human ATP-Binding Cassette, Sub-Family B, Member 1 ELISA kit

E01P0001 has been referenced in the below publications：

Correlations of ABCB1 gene C3435T polymorphism with serum and cerebrospinal fluid P-gp expression in Han people with epilepsy.

Relationship of P-glycoprotein expression of serum and cerebrospinal fluid and epilepsy drug-resistance in Hui and Han People with epilepsy.