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BlueGene Biotech Human Brain Derived Neurotrophic Factor ELISA kit (E01B0029)

E01B0029 Human Brain Derived Neurotrophic Factor ELISA kit

The Human Brain Derived Neurotrophic Factor ELISA kit can be used to identify samples from the human species. Brain Derived Neurotrophic Factor can also be called BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor.

Products

Specifications of Human Brain Derived Neurotrophic Factor ELISA kit

Product Information

Cat. No.

E01B0029

Product Name

Human Brain Derived Neurotrophic Factor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BDNF ELISA kit uses an anti-BDNF antibody and an BDNF-HRP conjugate in a competitive enzyme immunoassay method. BDNF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BDNF antibody binding site between BDNF from samples and BDNF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BDNF. Since the number of sites is limited, as more sites are occupied by BDNF from the sample, fewer sites are left to bind BDNF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDNF concentration in each sample is interpolated from this standard curve.


Quality Control on Human Brain Derived Neurotrophic Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BDNF and analogues was observed.


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Summary of the Assay Procedure for Human Brain Derived Neurotrophic Factor ELISA kit

Summary of the Assay Procedure for Human Brain Derived Neurotrophic Factor ELISA kit

Citations of Human Brain Derived Neurotrophic Factor ELISA kit

E01B0029 has been referenced in the below publications:

Association of brain-derived neurotrophic factor levels and depressive symptoms in young adults with acne vulgaris.

变应性鼻炎患者鼻黏膜中脑源性神经营养因子(BDNF)和IL-4表达的意义及相关性研究。

Association between brain-derived neurotrophic factor and severity of asthma in children.

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