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BlueGene Biotech Human Brain Natriuretic Peptide ELISA kit (E01B0452)

E01B0452 Human Brain Natriuretic Peptide ELISA kit

The Human Brain Natriuretic Peptide ELISA kit can be used to identify samples from the human species. Brain Natriuretic Peptide can also be called BNP, BNP 32, Brain natriuretic peptide 32, brain natriuretic peptide, Gamma brain natriuretic peptide, Natriuretic peptide brain type, Natriuretic peptide precursor B, Natriuretic peptides B, NPPB, NPPB protein, Iso-ANP, B-type natriuretic peptide.

Products

Specifications of Human Brain Natriuretic Peptide ELISA kit

Product Information

Cat. No.

E01B0452

Product Name

Human Brain Natriuretic Peptide ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BNP ELISA kit uses an anti-BNP antibody and an BNP-HRP conjugate in a competitive enzyme immunoassay method. BNP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BNP antibody binding site between BNP from samples and BNP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BNP. Since the number of sites is limited, as more sites are occupied by BNP from the sample, fewer sites are left to bind BNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BNP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Brain Natriuretic Peptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BNP and analogues was observed.


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Summary of the Assay Procedure for Human Brain Natriuretic Peptide ELISA kit

Summary of the Assay Procedure for Human Brain Natriuretic Peptide ELISA kit

Citations of Human Brain Natriuretic Peptide ELISA kit

E01B0452 has been referenced in the below publications:

Brain Volumetrics, Regional Cortical Thickness and Radiographic Findings in Adults with Cyanotic Congenital Heart Disease.

PLASMA ADIPONECTIN LEVELS AND ITS CORRELATION STUDY WITH BRAIN NATRIURETIC PEPTIDE, TUMOR NECROSIS FACTOR-a  AND INSULIN RESISTANCE IN PATIENTS WITH CHRONIC HEART FAILURE.

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