E01C0621 Human Carbonic Anhydrase II ELISA kit
The Human Carbonic Anhydrase II ELISA kit can be used to identify samples from the human species. Carbonic Anhydrase II can also be called CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282, Carbonic anhydrase II, carbonic anhydrase 2.
Product Information | |
Cat. No. | E01C0621 |
Product Name | Human Carbonic Anhydrase II ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CA2 ELISA kit uses an anti-CA2 antibody and an CA2-HRP conjugate in a competitive enzyme immunoassay method. CA2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CA2 antibody binding site between CA2 from samples and CA2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CA2. Since the number of sites is limited, as more sites are occupied by CA2 from the sample, fewer sites are left to bind CA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CA2 and analogues was observed. | |
E01C0621 has been referenced in the below publications：
Proteomics and Peptidomics Analysis of Lung Adenocarcinoma Malignant Pleural Effusion.
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