E01C0773 Human Carboxymethyl lysine ELISA kit
The Human Carboxymethyl lysine ELISA kit can be used to identify samples from the human species. Carboxymethyl lysine can also be called CML, N(epsilon)-(carboxymethyl)lysine, N(6)-Carboxymethyllysine, (2S)-2-Amino-6-[(carboxymethyl)amino]hexanoic acid.
Product Information | |
Cat. No. | E01C0773 |
Product Name | Human Carboxymethyl lysine ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 250 ng/mL | 1 vial |
STANDARD C (0.5mL) | 500 ng/mL | 1 vial |
STANDARD D (0.5mL) | 1000 ng/mL | 1 vial |
STANDARD E (0.5mL) | 2500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 5000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CML ELISA kit uses an anti-CML antibody and an CML-HRP conjugate in a competitive enzyme immunoassay method. CML-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CML antibody binding site between CML from samples and CML-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CML. Since the number of sites is limited, as more sites are occupied by CML from the sample, fewer sites are left to bind CML-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CML concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CML and analogues was observed. | |
E01C0773 has been referenced in the below publications：
Endoplasmic reticulum stress-dependent autophagy inhibits glycated high-density lipoprotein-induced macrophage apoptosis by inhibiting CHOP pathway.
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