E01C0666 Human Catecholamine ELISA kit
The Human Catecholamine ELISA kit can be used to identify samples from the human species. Catecholamine can also be called CA, catecholamine binding protein.
Specifications of Human Catecholamine ELISA kit
Product Information | |
Cat. No. | E01C0666 |
Product Name | Human Catecholamine ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CA ELISA kit uses an anti-CA antibody and an CA-HRP conjugate in a competitive enzyme immunoassay method. CA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CA antibody binding site between CA from samples and CA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CA. Since the number of sites is limited, as more sites are occupied by CA from the sample, fewer sites are left to bind CA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Catecholamine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Catecholamine ELISA kit
Citations of Human Catecholamine ELISA kit
E01C0666 has been referenced in the below publications：
The correlation between Helicobacter pylori infection and changes of immune function induced by Military stress.
