E01C0829 Human Chondroitin sulfate ELISA kit
The Human Chondroitin sulfate ELISA kit can be used to identify samples from the Human species. Chondroitin sulfate can also be called CS.
Specifications of Human Chondroitin sulfate ELISA kit
Product Information | |
Cat. No. | E01C0829 |
Product Name | Human Chondroitin sulfate ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CS ELISA kit uses an anti-CS antibody and an CS-HRP conjugate in a competitive enzyme immunoassay method. CS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CS antibody binding site between CS from samples and CS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CS. Since the number of sites is limited, as more sites are occupied by CS from the sample, fewer sites are left to bind CS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CS concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Chondroitin sulfate ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Chondroitin sulfate ELISA kit
Citations of Human Chondroitin sulfate ELISA kit
E01C0829 has been referenced in the below publications：
Acute Exacerbations of COPD Are Associated With Increased Expression of Heparan Sulfate and Chondroitin Sulfate in BAL.
