E01C0017 Human Collagen Type Ⅲ ELISA kit
The Human Collagen Type Ⅲ ELISA kit can be used to identify samples from the human species. Collagen Type Ⅲ can also be called COLIII, COL3A1, Collagen alpha 1(III) chain, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide.
Product Information | |
Cat. No. | E01C0017 |
Product Name | Human Collagen Type Ⅲ ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COLIII ELISA kit uses an anti-COLIII antibody and an COLIII-HRP conjugate in a competitive enzyme immunoassay method. COLIII-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-COLIII antibody binding site between COLIII from samples and COLIII-HRP conjugate, the intensity of the color is inversely proportional to the concentration of COLIII. Since the number of sites is limited, as more sites are occupied by COLIII from the sample, fewer sites are left to bind COLIII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COLIII concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COLIII and analogues was observed. | |
E01C0017 has been referenced in the below publications：
Role of up-regulation of THEM4 on collagen secretion in human renal tubular epithelial cells treated with high glucose.
Effect of THEM4 expression on collagen secretion in human embryo kidney epithelial cells treated with high glucose.
PTEN Regulates Renal Extracellular Matrix Deposit via Increased CTGF in Diabetes Mellitus.
Src Homology 2 Domain-Containing Inositol 5′-Phosphatase Ameliorates High Glucose-Induced Extracellular Matrix Deposition via the Phosphatidylinositol 3-Kinase/Protein Kinase B Pathway in Renal Tubular Epithelial Cells.
SnoN residue (1-366) attenuates hypertrophic scars through resistance to transforming growth factor-β1-induced degradation.
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