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  • bovine serum albumin elisa 1

BlueGene Biotech Human Complement Fragment 3a ELISA kit (E01C0658)

E01C0658 Human Complement Fragment 3a ELISA kit

The Human Complement Fragment 3a ELISA kit can be used to identify samples from the human species. Complement Fragment 3a can also be called complement C3, complement component 3.

Products

Specifications of Human Complement Fragment 3a ELISA kit

Product Information

Cat. No.

E01C0658

Product Name

Human Complement Fragment 3a ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

C3a ELISA kit uses an anti-C3a antibody and an C3a-HRP conjugate in a competitive enzyme immunoassay method. C3a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-C3a antibody binding site between C3a from samples and C3a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of C3a. Since the number of sites is limited, as more sites are occupied by C3a from the sample, fewer sites are left to bind C3a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3a concentration in each sample is interpolated from this standard curve.


Quality Control on Human Complement Fragment 3a ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between C3a and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Complement Fragment 3a ELISA kit

Summary of the Assay Procedure for Human Complement Fragment 3a ELISA kit

Citations of Human Complement Fragment 3a ELISA kit

E01C0658 has been referenced in the below publications:

Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.

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