E01C0088 Human Copeptin ELISA kit
The Human Copeptin ELISA kit can be used to identify samples from the human species. Copeptin can also be called CPP, CT-proAVP.
Specifications of Human Copeptin ELISA kit
Product Information | |
Cat. No. | E01C0088 |
Product Name | Human Copeptin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CPP ELISA kit uses an anti-CPP antibody and an CPP-HRP conjugate in a competitive enzyme immunoassay method. CPP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CPP antibody binding site between CPP from samples and CPP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CPP. Since the number of sites is limited, as more sites are occupied by CPP from the sample, fewer sites are left to bind CPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPP concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Copeptin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CPP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Copeptin ELISA kit
Citations of Human Copeptin ELISA kit
E01C0088 has been referenced in the below publications：
Exploration of the Diagnostic Value and the Disease Assessment of the Copeptin in the Community Acquired Pneumonia.
