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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Cyclin D1 ELISA kit (E01C0483)

E01C0483 Human Cyclin D1 ELISA kit

The Human Cyclin D1 ELISA kit can be used to identify samples from the human species. Cyclin D1 can also be called CCND1, BCL1, D11S287E, PRAD1, U21B31, cyclin D1.

Products

Specifications of Human Cyclin D1 ELISA kit

Product Information

Cat. No.

E01C0483

Product Name

Human Cyclin D1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CCND1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CCND1. Standards or samples are then added to the microtiter plate wells and CCND1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CCND1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CCND1 are added to each well to “sandwich” the CCND1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coCCND1nents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CCND1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCND1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Cyclin D1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CCND1 and analogues was observed.


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Summary of the Assay Procedure for Human Cyclin D1 ELISA kit

Summary of the Assay Procedure for Human Cyclin D1 ELISA kit

Citations of Human Cyclin D1 ELISA kit

E01C0483 has been referenced in the below publications:

NF-KB抑制剂对宫颈癌细胞株生物学特性的影响。

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