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  • bovine serum albumin elisa 1

BlueGene Biotech Human Cytochrome P450 1A1 ELISA kit (E01C2225)

E01C2225 Human Cytochrome P450 1A1 ELISA kit

The Human Cytochrome P450 1A1 ELISA kit can be used to identify samples from the human species. Cytochrome P450 1A1 can also be called CYP1A1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, CYPIA1, cytochrome P450 family 1 subfamily A member 1.

Products

Specifications of Human Cytochrome P450 1A1 ELISA kit

Product Information

Cat. No.

E01C2225

Product Name

Human Cytochrome P450 1A1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CYP1A1 ELISA kit uses an anti-CYP1A1 antibody and an CYP1A1-HRP conjugate in a competitive enzyme immunoassay method. CYP1A1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CYP1A1 antibody binding site between CYP1A1 from samples and CYP1A1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CYP1A1. Since the number of sites is limited, as more sites are occupied by CYP1A1 from the sample, fewer sites are left to bind CYP1A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP1A1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Cytochrome P450 1A1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CYP1A1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Cytochrome P450 1A1 ELISA kit

Summary of the Assay Procedure for Human Cytochrome P450 1A1 ELISA kit

Citations of Human Cytochrome P450 1A1 ELISA kit

E01C2225 has been referenced in the below publications:

Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development.

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