E01D0032 Human Diamine Oxidase ELISA kit
The Human Diamine Oxidase ELISA kit can be used to identify samples from the human species. Diamine Oxidase can also be called Amiloride Binding Protein 1, ABP1, AOC1, KAO, Histaminase, Kidney amine oxidase, Amiloride-sensitive amine oxidase, Amine oxidase copper domain-containing protein 1.
Product Information | |
Cat. No. | E01D0032 |
Product Name | Human Diamine Oxidase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DAO ELISA kit uses an anti-DAO antibody and an DAO-HRP conjugate in a competitive enzyme immunoassay method. DAO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DAO antibody binding site between DAO from samples and DAO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DAO. Since the number of sites is limited, as more sites are occupied by DAO from the sample, fewer sites are left to bind DAO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAO concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DAO and analogues was observed. | |
E01D0032 has been referenced in the below publications：
Effect of coupled plasma filtration adsorption combined with Continuous Hemofiltration Treatments on intestinal barrier function with SAP.
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