E01D0276 Human Disintegrin and metalloproteinase domain containing protein 17 ELISA kit
The Human Disintegrin and metalloproteinase domain containing protein 17 ELISA kit can be used to identify samples from the human species. Disintegrin and metalloproteinase domain containing protein 17 can also be called ADAM17, CD156B, CSVP, NISBD, NISBD1, TACE, ADAM metallopeptidase domain 17, TNF-alpha convertase, TNF-alpha-converting enzyme.
Product Information | |
Cat. No. | E01D0276 |
Product Name | Human Disintegrin and metalloproteinase domain containing protein 17 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ADAM17 ELISA kit uses an anti-ADAM17 antibody and an ADAM17-HRP conjugate in a competitive enzyme immunoassay method. ADAM17-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ADAM17 antibody binding site between ADAM17 from samples and ADAM17-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ADAM17. Since the number of sites is limited, as more sites are occupied by ADAM17 from the sample, fewer sites are left to bind ADAM17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM17 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ADAM17 and analogues was observed. | |
E01D0276 has been referenced in the below publications：
A novel marker of disease activity in Gaucher disease.
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