E01A0440 Human DNA (Double Stranded) ELISA Kit
The Human DNA (Double Stranded) ELISA Kit can be used to identify samples from the human species. DNA (Double Stranded) can also be called dsDNA.
Specifications of Human DNA (Double Stranded) ELISA Kit
Product Information | |
Cat. No. | E01A0440 |
Product Name | Human DNA (Double Stranded) ELISA Kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ds-DNA ELISA kit uses an anti-ds-DNA antibody and an ds-DNA-HRP conjugate in a competitive enzyme immunoassay method. ds-DNA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ds-DNA antibody binding site between ds-DNA from samples and ds-DNA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ds-DNA. Since the number of sites is limited, as more sites are occupied by ds-DNA from the sample, fewer sites are left to bind ds-DNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ds-DNA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human DNA (Double Stranded) ELISA Kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ds-DNA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human DNA (Double Stranded) ELISA Kit
Citations of Human DNA (Double Stranded) ELISA Kit
E01A0440 has been referenced in the below publications：
Laminin and collagen IV inclusion in immunoisolating microcapsules reduces cytokine-mediated cell death in human pancreatic islets.
