En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Dopamine Receptor D5 ELISA kit (E01D0056)

E01D0056 Human Dopamine Receptor D5 ELISA kit

The Human Dopamine Receptor D5 ELISA kit can be used to identify samples from the human species. Dopamine Receptor D5 can also be called D1BR, DRD5, DBDR, DRD1B, DRD1L2, dopamine receptor D5, D(1B) dopamine receptor, D(5) dopamine receptor, D1beta dopamine receptor, Dopamine D5 receptor.

Products

Specifications of Human Dopamine Receptor D5 ELISA kit

Product Information

Cat. No.

E01D0056

Product Name

Human Dopamine Receptor D5 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DRD5 ELISA kit uses an anti-DRD5 antibody and an DRD5-HRP conjugate in a competitive enzyme immunoassay method. DRD5-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DRD5 antibody binding site between DRD5 from samples and DRD5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DRD5. Since the number of sites is limited, as more sites are occupied by DRD5 from the sample, fewer sites are left to bind DRD5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD5 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Dopamine Receptor D5 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DRD5 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Dopamine Receptor D5 ELISA kit

Summary of the Assay Procedure for Human Dopamine Receptor D5 ELISA kit

Citations of Human Dopamine Receptor D5 ELISA kit

E01D0056 has been referenced in the below publications:

A study on the role of gene DRD5, MNX1 aberrant methylation in diagnosis of type 2 diabetic macrovascular complications.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.