E01D0325 Human Dysferlin ELISA kit
The Human Dysferlin ELISA kit can be used to identify samples from the human species. Dysferlin can also be called DYSF, FER1L1, LGMD2B, MMD1, dysferlin, LGMDR2, dystrophy-associated fer-1-like protein.
Specifications of Human Dysferlin ELISA kit
Product Information | |
Cat. No. | E01D0325 |
Product Name | Human Dysferlin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| DYSF ELISA kit uses an anti-DYSF antibody and an DYSF-HRP conjugate in a competitive enzyme immunoassay method. DYSF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DYSF antibody binding site between DYSF from samples and DYSF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DYSF. Since the number of sites is limited, as more sites are occupied by DYSF from the sample, fewer sites are left to bind DYSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DYSF concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Dysferlin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DYSF and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Dysferlin ELISA kit
Citations of Human Dysferlin ELISA kit
E01D0325 has been referenced in the below publications：
Expression and Significance of Dysferlin and Affixin in Preeclampsia.
