E01E0141 Human Endothelial leukocyte adhesion molecule 1 ELISA kit
The Human Endothelial leukocyte adhesion molecule 1 ELISA kit can be used to identify samples from the human species. Endothelial leukocyte adhesion molecule 1 can also be called SELE, CD62E, ELAM, ELAM1, ELAM-1, ESEL, LECAM2, selectin E, selectin-e, E-selectin, CD62 antigen-like family member E, leukocyte-endothelial cell adhesion molecule 2.
Product Information | |
Cat. No. | E01E0141 |
Product Name | Human Endothelial leukocyte adhesion molecule 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ELAM 1 ELISA kit uses an anti-ELAM 1 antibody and an ELAM 1-HRP conjugate in a competitive enzyme immunoassay method. ELAM 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ELAM 1 antibody binding site between ELAM 1 from samples and ELAM 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ELAM 1. Since the number of sites is limited, as more sites are occupied by ELAM 1 from the sample, fewer sites are left to bind ELAM 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELAM 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ELAM 1 and analogues was observed. | |
E01E0141 has been referenced in the below publications：
Experimental effects of Drynaria fortunei on alopecia areata induced by cyclophosphamide in mice.
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