E01E0028 Human Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA kit
The Human Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA kit can be used to identify samples from the human species. Epidermal Growth Factor Receptor 2 Extracellular Domain can also be called HER 2 ECD.
Product Information | |
Cat. No. | E01E0028 |
Product Name | Human Epidermal Growth Factor Receptor 2 Extracellular Domain ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HER2ECD ELISA kit uses an anti-HER2ECD antibody and an HER2ECD-HRP conjugate in a competitive enzyme immunoassay method. HER2ECD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HER2ECD antibody binding site between HER2ECD from samples and HER2ECD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HER2ECD. Since the number of sites is limited, as more sites are occupied by HER2ECD from the sample, fewer sites are left to bind HER2ECD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HER2ECD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HER2ECD and analogues was observed. | |
E01E0028 has been referenced in the below publications：
Her-2/neu expression in serum and tumor tissue and relation between Her-2/neu expression and clinicopathologic features in patients with gastric cancer.
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