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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Fatty Acid Binding Protein 1, Liver ELISA Kit (E01L0300)

E01L0300 Human Fatty Acid Binding Protein 1, Liver ELISA Kit

The Human Fatty Acid Binding Protein 1, Liver ELISA Kit can be used to identify samples from the human species. Fatty Acid Binding Protein 1, Liver can also be called LFABP, FABP1, FABPL, L-FABP, fatty acid binding protein 1.

Products

Specifications of Human Fatty Acid Binding Protein 1, Liver ELISA Kit

Product Information

Cat. No.

E01L0300

Product Name

Human Fatty Acid Binding Protein 1, Liver ELISA Kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FABP1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FABP1. Standards or samples are then added to the microtiter plate wells and FABP1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FABP1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FABP1 are added to each well to “sandwich” the FABP1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FABP1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Fatty Acid Binding Protein 1, Liver ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FABP1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Fatty Acid Binding Protein 1, Liver ELISA Kit

Summary of the Assay Procedure for Human Fatty Acid Binding Protein 1, Liver ELISA Kit

Citations of Human Fatty Acid Binding Protein 1, Liver ELISA Kit

E01L0300 has been referenced in the below publications:

Intrarenal Liver-Type Fatty Acid Binding Protein effect of Ig A Nephropathy progress.

Early Biomarkers of Renal Damage in Relation to Arterial Stiffness and Inflammation in Male Coronary Artery Disease Patients.

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