Human Fatty Acid Synthase ELISA kit (E01F0043) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Fatty Acid Synthase ELISA kit (E01F0043)

E01F0043 Human Fatty Acid Synthase ELISA kit

The Human Fatty Acid Synthase ELISA kit can be used to identify samples from the human species. Fatty Acid Synthase can also be called CD95 (phospho Y232), CD95 (phospho Tyr232), p-CD95 (Tyr232), FAS (phospho Y232), FAS (phospho Tyr232), p-FAS (Tyr232), ALPS 1A, ALPS1A, APO 1, Apo 1 antigen, APO 1 cell surface antigen, Apo-1 antigen, APO1, Apo1 antigen, APO1 cell surface antigen, Apoptosis antigen 1, Apoptosis mediating surface antigen FAS, Apoptosis-mediating surface antigen FAS, APT 1, APT1, CD 95, CD 95 antigen, Delta Fas, Delta Fas/APO 1/CD95, FAS 1, FAS 827dupA, Fas AMA, FAS, FAS Antigen, FAS1, FASLG receptor, FASTM, TNF receptor superfamily, member 6, TNFRSF 6, TNFRSF6, Tumor necrosis factor receptor superfamily member 6.

Products

Specifications of Human Fatty Acid Synthase ELISA kit

Product Information
Cat. No.	E01F0043
Product Name	Human Fatty Acid Synthase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50ng/mL
Sensitivity	0.1 ng/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

FAS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FAS. Standards or samples are then added to the microtiter plate wells and FAS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FAS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FAS are added to each well to “sandwich” the FAS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FAS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAS concentration in each sample is interpolated from this standard curve.

Quality Control on Human Fatty Acid Synthase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between FAS and analogues was observed.