E01F0010 Human Ferritin ELISA kit
The Human Ferritin ELISA kit can be used to identify samples from the human species. Ferritin can also be called FE.
Specifications of Human Ferritin ELISA kit
Product Information | |
Cat. No. | E01F0010 |
Product Name | Human Ferritin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FE ELISA kit uses an anti-FE antibody and an FE-HRP conjugate in a competitive enzyme immunoassay method. FE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FE antibody binding site between FE from samples and FE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FE. Since the number of sites is limited, as more sites are occupied by FE from the sample, fewer sites are left to bind FE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Ferritin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FE and analogues was observed. | |
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Summary of the Assay Procedure for Human Ferritin ELISA kit
Citations of Human Ferritin ELISA kit
E01F0010 has been referenced in the below publications：
Study on the relationship between serum ferritin,Transferrin, hepcidin and type 2 diabetes.
