Human Fibronectin ELISA kit (E01F0004) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Fibronectin ELISA kit (E01F0004)

E01F0004 Human Fibronectin ELISA kit

The Human Fibronectin ELISA kit can be used to identify samples from the human species. Fibronectin can also be called FN, CIG, Cold insoluble globulin, ED B, ED-B, fibronectin 1, FINC, FN 1 FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor, MSF, Transformation sensitive protein, Fibronectin, Cold-insoluble globulin.

Products

Specifications of Human Fibronectin ELISA kit

Product Information
Cat. No.	E01F0004
Product Name	Human Fibronectin ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 ng/ml
Sensitivity	1.0 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	50 ng/mL	1 vial
STANDARD C (0.5mL)	100 ng/mL	1 vial
STANDARD D (0.5mL)	250 ng/mL	1 vial
STANDARD E (0.5mL)	500 ng/mL	1 vial
STANDARD F (0.5mL)	1000 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

FN ELISA kit uses an anti-FN antibody and an FN-HRP conjugate in a competitive enzyme immunoassay method. FN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FN antibody binding site between FN from samples and FN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FN. Since the number of sites is limited, as more sites are occupied by FN from the sample, fewer sites are left to bind FN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FN concentration in each sample is interpolated from this standard curve.

Quality Control on Human Fibronectin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between FN and analogues was observed.