E01F0001 Human Follicle Stimulating Hormone ELISA kit
The Human Follicle Stimulating Hormone ELISA kit can be used to identify samples from the human species. Follicle Stimulating Hormone can also be called Follicle Stimulating Hormone, Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin beta chain precursor, Follitropin subunit beta, FSH B, FSH-B, FSH-beta, FSHB, FSHbeta .
Product Information | |
Cat. No. | E01F0001 |
Product Name | Human Follicle Stimulating Hormone ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 mIU/ml |
Sensitivity | 1.0 mIU/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mIU/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 mIU/ml | 1 vial |
STANDARD C (0.5mL) | 10 mIU/ml | 1 vial |
STANDARD D (0.5mL) | 25 mIU/ml | 1 vial |
STANDARD E (0.5mL) | 50 mIU/ml | 1 vial |
STANDARD F (0.5mL) | 100 mIU/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FSH ELISA kit uses an anti-FSH antibody and an FSH-HRP conjugate in a competitive enzyme immunoassay method. FSH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FSH antibody binding site between FSH from samples and FSH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FSH. Since the number of sites is limited, as more sites are occupied by FSH from the sample, fewer sites are left to bind FSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSH concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FSH and analogues was observed. | |
E01F0001 has been referenced in the below publications：
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