E01I0057 Human Indoleamine 2,3 Dioxygenase ELISA kit
The Human Indoleamine 2,3 Dioxygenase ELISA kit can be used to identify samples from the human species. Indoleamine 2,3 Dioxygenase can also be called Indoleamine 2, 3-dioxygenase , 3-dioxygenase, IDO 1, INDO, Indole 2 3 dioxygenase, indoleamine 2 3 dioxygenase 1, indoleamine 2 3 dioxygenase, Indoleamine pyrrole 2 3 dioxygenase, Indoleamine-pyrrole 2.
Product Information | |
Cat. No. | E01I0057 |
Product Name | Human Indoleamine 2,3 Dioxygenase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IDO ELISA kit uses an anti-IDO antibody and an IDO-HRP conjugate in a competitive enzyme immunoassay method. IDO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IDO antibody binding site between IDO from samples and IDO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IDO. Since the number of sites is limited, as more sites are occupied by IDO from the sample, fewer sites are left to bind IDO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDO concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IDO and analogues was observed. | |
E01I0057 has been referenced in the below publications：
CCL2 deficient mesenchymal stem cells fail to establish long-lasting contact with T cells and no longer ameliorate lupus symptoms.
Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke model.
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