E01G0033 Human Galanin ELISA kit
The Human Galanin ELISA kit can be used to identify samples from the human species. Galanin can also be called GAL 1, Galanin precursor, GALN, GLNN, GMAP, GAL, GAL-GMAP, ETL8, galanin and GMAP prepropeptide.
Specifications of Human Galanin ELISA kit
Product Information | |
Cat. No. | E01G0033 |
Product Name | Human Galanin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GAL ELISA kit uses an anti-GAL antibody and an GAL-HRP conjugate in a competitive enzyme immunoassay method. GAL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GAL antibody binding site between GAL from samples and GAL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GAL. Since the number of sites is limited, as more sites are occupied by GAL from the sample, fewer sites are left to bind GAL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Galanin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GAL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Galanin ELISA kit
Citations of Human Galanin ELISA kit
E01G0033 has been referenced in the below publications：
Goal-directed Fluid Therapy Effects on Bowel Movement Function in Patients with Gastrointestinal Tumor.
中药复方治疗抑郁症的临床研究。
