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BlueGene Biotech Human Galectin 9 ELISA kit (E01G0073)

E01G0073 Human Galectin 9 ELISA kit

The Human Galectin 9 ELISA kit can be used to identify samples from the human species. Galectin 9 can also be called GAL9, galectin9, LGALS9, HOMHD21, HUAT, LGALS9A, galectin 9, Tumor antigen HOM-HD-21, Ecalectin, Gal-9, Galectin-9, LGALS9, Lectin galactoside binding soluble 9.

Products

Specifications of Human Galectin 9 ELISA kit

Product Information

Cat. No.

E01G0073

Product Name

Human Galectin 9 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GAL9 ELISA kit uses an anti-GAL9 antibody and an GAL9-HRP conjugate in a competitive enzyme immunoassay method. GAL9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GAL9 antibody binding site between GAL9 from samples and GAL9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GAL9. Since the number of sites is limited, as more sites are occupied by GAL9 from the sample, fewer sites are left to bind GAL9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL9 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Galectin 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GAL9 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Galectin 9 ELISA kit

Summary of the Assay Procedure for Human Galectin 9 ELISA kit

Citations of Human Galectin 9 ELISA kit

E01G0073 has been referenced in the below publications:

Soluble costimulatory molecule sTim3 regulates the differentiation of Th1 and Th2 in patients with unexplained recurrent spontaneous abortion.

Peripheral Blood TIM-3 Positive NK and CD8+ T Cells throughout Pregnancy: TIM-3/Galectin-9 Interaction and Its Possible Role during Pregnancy.

The Diagnostic Accuracy of Galectin-9 for Diagnosis of Endometriosis in Comparison with Laparoscopy.

Plasma Galectin-9 Concentrations in Normal and Diseased Condition.

Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders.

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