E01G0360 Human Glucocorticoid receptor Alpha ELISA kit
The Human Glucocorticoid receptor Alpha ELISA kit can be used to identify samples from the human species. Glucocorticoid receptor Alpha can also be called GR Alpha, GR-A, GR-α, Gra.
Specifications of Human Glucocorticoid receptor Alpha ELISA kit
Product Information | |
Cat. No. | E01G0360 |
Product Name | Human Glucocorticoid receptor Alpha ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GR Alpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GR Alpha. Standards or samples are then added to the microtiter plate wells and GR Alpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GR Alpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GR Alpha are added to each well to “sandwich” the GR Alpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coGR Alphanents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GR Alpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GR Alpha concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Glucocorticoid receptor Alpha ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GR Alpha and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Glucocorticoid receptor Alpha ELISA kit
Citations of Human Glucocorticoid receptor Alpha ELISA kit
E01G0360 has been referenced in the below publications：
Study on the glucocorticoid receptors in patients with Kawasaki disease.
