Human Heat Shock Protein 70 ELISA kit (E01H0022) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Heat Shock Protein 70 ELISA kit (E01H0022)

Signal transduction

E01H0022 Human Heat Shock Protein 70 ELISA kit

The Human Heat Shock Protein 70 ELISA kit can be used to identify samples from the human species. Heat Shock Protein 70 can also be called Hsp70, DnaK, 70 Kilodalton Heat Shock Proteins.

Products

Specifications of Human Heat Shock Protein 70 ELISA kit

Product Information
Cat. No.	E01H0022
Product Name	Human Heat Shock Protein 70 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

HSP70 ELISA kit uses an anti-HSP70 antibody and an HSP70-HRP conjugate in a competitive enzyme immunoassay method. HSP70-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HSP70 antibody binding site between HSP70 from samples and HSP70-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HSP70. Since the number of sites is limited, as more sites are occupied by HSP70 from the sample, fewer sites are left to bind HSP70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Heat Shock Protein 70 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between HSP70 and analogues was observed.