E01H1362 Human hemoglobin ELISA kit
The Human hemoglobin ELISA kit can be used to identify samples from the human species. hemoglobin can also be called HB, Hb, Hgb, haemoglobin, Hgb, Haemoglobin, heterotetramer (αβ)2.
Specifications of Human hemoglobin ELISA kit
Product Information | |
Cat. No. | E01H1362 |
Product Name | Human hemoglobin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ug/mL |
Sensitivity | 1.0 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 25 ug/mL | 1 vial |
STANDARD C (0.5mL) | 50 ug/mL | 1 vial |
STANDARD D (0.5mL) | 100 ug/mL | 1 vial |
STANDARD E (0.5mL) | 250 ug/mL | 1 vial |
STANDARD F (0.5mL) | 500 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Hb ELISA kit uses an anti-Hb antibody and an Hb-HRP conjugate in a competitive enzyme immunoassay method. Hb-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Hb antibody binding site between Hb from samples and Hb-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Hb. Since the number of sites is limited, as more sites are occupied by Hb from the sample, fewer sites are left to bind Hb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hb concentration in each sample is interpolated from this standard curve. |
Quality Control on Human hemoglobin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Hb and analogues was observed. | |
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Summary of the Assay Procedure for Human hemoglobin ELISA kit
Citations of Human hemoglobin ELISA kit
E01H1362 has been referenced in the below publications：
Effect of different hemodialysis modalities on resistin and nutritional status.
