E01H0100 Human Heparanase ELISA kit
The Human Heparanase ELISA kit can be used to identify samples from the human species. Heparanase can also be called HPSE, HPA, HPA1, HPR1, HPSE1, HSE1, heparanase.
Specifications of Human Heparanase ELISA kit
Product Information | |
Cat. No. | E01H0100 |
Product Name | Human Heparanase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HPA ELISA kit uses an anti-HPA antibody and an HPA-HRP conjugate in a competitive enzyme immunoassay method. HPA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HPA antibody binding site between HPA from samples and HPA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HPA. Since the number of sites is limited, as more sites are occupied by HPA from the sample, fewer sites are left to bind HPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Heparanase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HPA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Heparanase ELISA kit
Citations of Human Heparanase ELISA kit
E01H0100 has been referenced in the below publications：
Serum heparanase levels: A protective marker against digital ulcers in patients with systemic sclerosis.
Combination of drug-conjugated SWCNT nanocarriers for efficient therapy of cancer stem cells in a breast cancer animal model.
