E01L0068 Human Lysozyme/Muramidase ELISA kit
The Human Lysozyme/Muramidase ELISA kit can be used to identify samples from the human species. Lysozyme/Muramidase can also be called LYZ, Renal Amyloidosis, N-Acetylmuramide Glycanhydrolase, Muramidase, 1,4-beta-N-acetylmuramidase C.
Product Information | |
Cat. No. | E01L0068 |
Product Name | Human Lysozyme/Muramidase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 µg/mL |
Sensitivity | 0.1 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µg/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 µg/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 µg/mL | 1 vial |
STANDARD D (0.5mL) | 10 µg/mL | 1 vial |
STANDARD E (0.5mL) | 25 µg/mL | 1 vial |
STANDARD F (0.5mL) | 50 µg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LZM ELISA kit uses an anti-LZM antibody and an LZM-HRP conjugate in a competitive enzyme immunoassay method. LZM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LZM antibody binding site between LZM from samples and LZM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LZM. Since the number of sites is limited, as more sites are occupied by LZM from the sample, fewer sites are left to bind LZM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LZM concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LZM and analogues was observed. | |
E01L0068 has been referenced in the below publications：
Gold nanoclusters-based chemiluminescence resonance energy transfer method for sensitive and label-free detection of trypsin.
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