E01H1404 Human High-temperature requirement factor A4 ELISA kit
The Human High-temperature requirement factor A4 ELISA kit can be used to identify samples from the human species. High-temperature requirement factor A4 can also be called HTRA4.
Specifications of Human High-temperature requirement factor A4 ELISA kit
Product Information | |
Cat. No. | E01H1404 |
Product Name | Human High-temperature requirement factor A4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HTRA4 ELISA kit uses an anti-HTRA4 antibody and an HTRA4-HRP conjugate in a competitive enzyme immunoassay method. HTRA4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HTRA4 antibody binding site between HTRA4 from samples and HTRA4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HTRA4. Since the number of sites is limited, as more sites are occupied by HTRA4 from the sample, fewer sites are left to bind HTRA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HTRA4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human High-temperature requirement factor A4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HTRA4 and analogues was observed. | |
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Summary of the Assay Procedure for Human High-temperature requirement factor A4 ELISA kit
Citations of Human High-temperature requirement factor A4 ELISA kit
E01H1404 has been referenced in the below publications：
Study of Htr A4 and Syncytin-1 in Preeclampsia.
