E01H1481 Human Homeobox protein Hox-A9 ELISA kit
The Human Homeobox protein Hox-A9 ELISA kit can be used to identify samples from the human species. Homeobox protein Hox-A9 can also be called HOXA9, ABD-B, HOX1, HOX1.7, HOX1G, homeobox A9.
Specifications of Human Homeobox protein Hox-A9 ELISA kit
Product Information | |
Cat. No. | E01H1481 |
Product Name | Human Homeobox protein Hox-A9 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HOXA9 ELISA kit uses an anti-HOXA9 antibody and an HOXA9-HRP conjugate in a competitive enzyme immunoassay method. HOXA9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HOXA9 antibody binding site between HOXA9 from samples and HOXA9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HOXA9. Since the number of sites is limited, as more sites are occupied by HOXA9 from the sample, fewer sites are left to bind HOXA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HOXA9 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Homeobox protein Hox-A9 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HOXA9 and analogues was observed. | |
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Summary of the Assay Procedure for Human Homeobox protein Hox-A9 ELISA kit
Citations of Human Homeobox protein Hox-A9 ELISA kit
E01H1481 has been referenced in the below publications：
Expression of HOXA9 in follicular fluid and luteinized granulosa cells of Polycystic Ovary Syndrome and its correlation with IGFs.
