E01I0012 Human Inhibin ELISA kit
The Human Inhibin ELISA kit can be used to identify samples from the human species. Inhibin can also be called INH.
Specifications of Human Inhibin ELISA kit
Product Information | |
Cat. No. | E01I0012 |
Product Name | Human Inhibin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
INH ELISA kit uses an anti-INH antibody and an INH-HRP conjugate in a competitive enzyme immunoassay method. INH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-INH antibody binding site between INH from samples and INH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of INH. Since the number of sites is limited, as more sites are occupied by INH from the sample, fewer sites are left to bind INH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INH concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Inhibin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between INH and analogues was observed. | |
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Summary of the Assay Procedure for Human Inhibin ELISA kit
Citations of Human Inhibin ELISA kit
E01I0012 has been referenced in the below publications：
Polyinosinic-polycytidylic acid induces innate immune responses via Toll like receptor 3 in human ovarian granulosa cells.
