Human Insulin like growth factor 2 ELISA kit (E01I0300) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Insulin like growth factor 2 ELISA kit (E01I0300)

E01I0300 Human Insulin like growth factor 2 ELISA kit

The Human Insulin like growth factor 2 ELISA kit can be used to identify samples from the human species. Insulin like growth factor 2 can also be called IGF 2, IGF-2, IGF2, C11orf43, GRDF, IGF-II, PP9974, insulin like growth factor 2, SRS3.

Products

Specifications of Human Insulin like growth factor 2 ELISA kit

Product Information
Cat. No.	E01I0300
Product Name	Human Insulin like growth factor 2 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IGF2 ELISA kit uses an anti-IGF2 antibody and an IGF2-HRP conjugate in a competitive enzyme immunoassay method. IGF2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IGF2 antibody binding site between IGF2 from samples and IGF2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IGF2. Since the number of sites is limited, as more sites are occupied by IGF2 from the sample, fewer sites are left to bind IGF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF2 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Insulin like growth factor 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IGF 2 and analogues was observed.