E01I0009 Human Insulin like Growth Factor1 ELISA kit
The Human Insulin like Growth Factor1 ELISA kit can be used to identify samples from the human species. Insulin like Growth Factor1 can also be called IGF1, IBP1, IGF IA, IGF IB, IGFI, IGFIA, Insulin Like Growth Factor 1, Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, MGF, Mechano growth factor, MGF, Somatomedia C, Somatomedin C.
Product Information | |
Cat. No. | E01I0009 |
Product Name | Human Insulin like Growth Factor1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IGF1 ELISA kit uses an anti-IGF1 antibody and an IGF1-HRP conjugate in a competitive enzyme immunoassay method. IGF1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IGF1 antibody binding site between IGF1 from samples and IGF1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IGF1. Since the number of sites is limited, as more sites are occupied by IGF1 from the sample, fewer sites are left to bind IGF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IGF1 and analogues was observed. | |
E01I0009 has been referenced in the below publications：
Cadaveric cardiosphere-derived cells can maintain regenerative capacity and improve the heart function of cardiomyopathy.
An efficacy study of acupuncture on facial acne vulgaris, serum IGF-1 and DHEA levels as well as its implications on depressive and anxiety symptoms.
Expression of HOXA9 in follicular fluid and luteinized granulosa cells of Polycystic Ovary Syndrome and its correlation with IGFs.
Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-I/PI3K/Akt Pathways.
Increased placental IGF-1/mTOR activity in macrosomia born to women with gestational diabetes.
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