E01I0394 Human Ischemia Modified Albumin ELISA kit
The Human Ischemia Modified Albumin ELISA kit can be used to identify samples from the human species. Ischemia Modified Albumin can also be called IMA.
Specifications of Human Ischemia Modified Albumin ELISA kit
Product Information | |
Cat. No. | E01I0394 |
Product Name | Human Ischemia Modified Albumin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IMA ELISA kit uses an anti-IMA antibody and an IMA-HRP conjugate in a competitive enzyme immunoassay method. IMA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IMA antibody binding site between IMA from samples and IMA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IMA. Since the number of sites is limited, as more sites are occupied by IMA from the sample, fewer sites are left to bind IMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Ischemia Modified Albumin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IMA and analogues was observed. | |
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Summary of the Assay Procedure for Human Ischemia Modified Albumin ELISA kit
Citations of Human Ischemia Modified Albumin ELISA kit
E01I0394 has been referenced in the below publications：
Effect of ischemic postconditioning on myocardial injury in patients undergoing cardiac valve replacement under cardiopulmonary bypass.
Investigation on the complementation significance of combined cTnI,IMA and hs-CRP determination in coronary heart disease.
