E01K0076 Human keratan sulfate ELISA kit
The Human keratan sulfate ELISA kit can be used to identify samples from the human species. Keratan sulfate can also be called KS, Keratosulfate, Keratan sulfate.
Specifications of Human keratan sulfate ELISA kit
Product Information | |
Cat. No. | E01K0076 |
Product Name | Human keratan sulfate ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KS ELISA kit uses an anti-KS antibody and an KS-HRP conjugate in a competitive enzyme immunoassay method. KS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KS antibody binding site between KS from samples and KS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KS. Since the number of sites is limited, as more sites are occupied by KS from the sample, fewer sites are left to bind KS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KS concentration in each sample is interpolated from this standard curve. |
Quality Control on Human keratan sulfate ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human keratan sulfate ELISA kit
Citations of Human keratan sulfate ELISA kit
E01K0076 has been referenced in the below publications：
Effects of a 15-month anti-TNF-α treatment on plasma levels of glycosaminoglycans in women with rheumatoid arthritis.
Variation of semen KLK2, KLK3, KLK5 in patients of delayed semen liquefaction.
Cartilage metabolic markers and antioxidants: its correlation in osteoarthritic patients of sikkim.
