En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human L selectin ELISA kit (E01L0002)

E01L0002 Human L selectin ELISA kit

The Human L selectin ELISA kit can be used to identify samples from the human species. L selectin can also be called L selectin, L-selectin, SELL, CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1, selectin L, CD62 antigen-like family member L, LAM-1, Leukocyte adhesion molecule 1, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, gp90-MEL.

Products

Specifications of Human L selectin ELISA kit

Product Information

Cat. No.

E01L0002

Product Name

Human L selectin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

L selectin ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for L selectin. Standards or samples are then added to the microtiter plate wells and L selectin if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of L selectin present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for L selectin are added to each well to “sandwich” the L selectin immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coL selectinnents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain L selectin and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L selectin concentration in each sample is interpolated from this standard curve.


Quality Control on Human L selectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between L selectin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human L selectin ELISA kit

Summary of the Assay Procedure for Human L selectin ELISA kit

Citations of Human L selectin ELISA kit

E01L0002 has been referenced in the below publications:

Effects of recombinant human growth hormone on the expression of CD47, L-selectin and advanced oxidation protein products in elderly patients with multiple organ dysfunction syndrome.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.