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BlueGene Biotech Human Latent membrane protein 1 ELISA kit (E01L0243)

E01L0243 Human Latent membrane protein 1 ELISA kit

The Human Latent membrane protein 1 ELISA kit can be used to identify samples from the human species. Latent membrane protein 1 can also be called LMP 1, LMP-1.

Products

Specifications of Human Latent membrane protein 1 ELISA kit

Product Information

Cat. No.

E01L0243

Product Name

Human Latent membrane protein 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LMP 1 ELISA kit uses an anti-LMP 1 antibody and an LMP 1-HRP conjugate in a competitive enzyme immunoassay method. LMP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LMP 1 antibody binding site between LMP 1 from samples and LMP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LMP 1. Since the number of sites is limited, as more sites are occupied by LMP 1 from the sample, fewer sites are left to bind LMP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LMP 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Latent membrane protein 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LMP 1 and analogues was observed.


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Summary of the Assay Procedure for Human Latent membrane protein 1 ELISA kit

Summary of the Assay Procedure for Human Latent membrane protein 1 ELISA kit

Citations of Human Latent membrane protein 1 ELISA kit

E01L0243 has been referenced in the below publications:

The expression of LMP-1 gene in juve nile lupus nephritis.

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