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  • bovine serum albumin elisa 1

BlueGene Biotech Human Latent transforming growth factor β binding protein 1 ELISA kit (E01L0245)

E01L0245 Human Latent transforming growth factor β binding protein 1 ELISA kit

The Human Latent transforming growth factor β binding protein 1 ELISA kit can be used to identify samples from the human species. Latent transforming growth factor β binding protein 1 can also be called TGF-beta1-BP-1, Transforming growth factor beta-1-binding protein 1.

Products

Specifications of Human Latent transforming growth factor β binding protein 1 ELISA kit

Product Information

Cat. No.

E01L0245

Product Name

Human Latent transforming growth factor β binding protein 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LTBP1 ELISA kit uses an anti-LTBP1 antibody and an LTBP1-HRP conjugate in a competitive enzyme immunoassay method. LTBP1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LTBP1 antibody binding site between LTBP1 from samples and LTBP1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LTBP1. Since the number of sites is limited, as more sites are occupied by LTBP1 from the sample, fewer sites are left to bind LTBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTBP1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Latent transforming growth factor β binding protein 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LTBP1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Latent transforming growth factor β binding protein 1 ELISA kit

Summary of the Assay Procedure for Human Latent transforming growth factor β binding protein 1 ELISA kit

Citations of Human Latent transforming growth factor β binding protein 1 ELISA kit

E01L0245 has been referenced in the below publications:

Latent transforming growth factor-beta binding protein-1 in circulating plasma as a novel biomarker for early detection of hepatocellular carcinoma.

Prognostic stratification of patients with lung,breast and liver cancers by EMT-related gene-expression profiling in tumor-adjacent tissues.

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