E01L0037 Human Leukotriene B4 ELISA kit
The Human Leukotriene B4 ELISA kit can be used to identify samples from the human species. Leukotriene B4 can also be called LTB4, LT-B4.
Specifications of Human Leukotriene B4 ELISA kit
Product Information | |
Cat. No. | E01L0037 |
Product Name | Human Leukotriene B4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LTB4 ELISA kit uses an anti-LTB4 antibody and a LTB4-HRP conjugate in a competitive enzyme immunoassay method. LTB4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LTB4 antibody binding site between LTB4 from samples and LTB4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LTB4. Since the number of sites is limited, as more sites are occupied by LTB4 from the sample, fewer sites are left to bind LTB4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTB4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Leukotriene B4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LTB4 and analogues was observed. | |
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Summary of the Assay Procedure for Human Leukotriene B4 ELISA kit
Citations of Human Leukotriene B4 ELISA kit
E01L0037 has been referenced in the below publications：
Clinical Observation of Concentration Variation of Conleukotriene B4 and Tumornecrosis factor-a During Different Periods of Bronchiectasis and its Relevance with Lung Function.
Effect of Yangyin Yifei Fang on Leukotrience B4 Level in Patients with Chronic Obstructive Pulmonary Disease in Stable Stage of Qi and Yin Deficiency Type.
