E01L0207 Human Lipoarabinomannan ELISA kit
The Human Lipoarabinomannan ELISA kit can be used to identify samples from the human species. Lipoarabinomannan can also be called LAM.
Specifications of Human Lipoarabinomannan ELISA kit
Product Information | |
Cat. No. | E01L0207 |
Product Name | Human Lipoarabinomannan ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LAM ELISA kit uses an anti-LAM antibody and an LAM-HRP conjugate in a competitive enzyme immunoassay method. LAM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LAM antibody binding site between LAM from samples and LAM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LAM. Since the number of sites is limited, as more sites are occupied by LAM from the sample, fewer sites are left to bind LAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LAM concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Lipoarabinomannan ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LAM and analogues was observed. | |
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Summary of the Assay Procedure for Human Lipoarabinomannan ELISA kit
Citations of Human Lipoarabinomannan ELISA kit
E01L0207 has been referenced in the below publications：
Values of Early Secretory Antigenic Target 6 and Lipoarabinomannan in Diagnosis of Tuberculous Meningitis.
