E01P0066 Human Protein C Inhibitor ELISA kit
The Human Protein C Inhibitor ELISA kit can be used to identify samples from the human species. Protein C Inhibitor can also be called PCOL-I, PC-I, PC1, Type I Procollagen.
Specifications of Human Protein C Inhibitor ELISA kit
Product Information | |
Cat. No. | E01P0066 |
Product Name | Human Protein C Inhibitor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PCI ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PCI antibody and an PCI-HRP conjugate. The assay sample and buffer are incubated together with PCI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCI concentration since PCI from samples and PCI-HRP conjugate compete for the anti-PCI antibody binding site. Since the number of sites is limited, as more sites are occupied by PCI from the sample, fewer sites are left to bind PCI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCI concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Protein C Inhibitor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PCI and analogues was observed. | |
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Summary of the Assay Procedure for Human Protein C Inhibitor ELISA kit
Citations of Human Protein C Inhibitor ELISA kit
E01P0066 has been referenced in the below publications：
Increased Serpin A5 levels in the cervicovaginal fluid of HIV-1 exposed seronegatives suggest that a subtle balance between serine proteases and their inhibitors may determine susceptibility to HIV-1 infection.
