E01M0329 Human Matrix metalloproteinase 9 ELISA kit
The Human Matrix metalloproteinase 9 ELISA kit can be used to identify samples from the human species. Matrix metalloproteinase 9 can also be called MMP 9, Matrix metalloproteinase-9 precursor, 92 kDa type IV. Collagenase, 92 kDa gelatinase, Gelatinase B, GELB.
Product Information | |
Cat. No. | E01M0329 |
Product Name | Human Matrix metalloproteinase 9 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MMP 9 ELISA kit uses an anti-MMP 9 antibody and an MMP 9-HRP conjugate in a competitive enzyme immunoassay method. MMP 9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MMP 9 antibody binding site between MMP 9 from samples and MMP 9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MMP 9. Since the number of sites is limited, as more sites are occupied by MMP 9 from the sample, fewer sites are left to bind MMP 9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 9 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MMP 9 and analogues was observed. | |
E01M0329 has been referenced in the below publications：
Overexpression of CCN3 Inhibits Inflammation and Progression of Atherosclerosis in Apolipoprotein EDeficient Mice.
The expression of HMGB1 in patients with acute coronary syndrome and effects of loading dose of atorvastatin in these patients during PCI perioperative period.
Expression and significance of YKL-40 protein in the supernatant of prostate cancer cells.
Effects of atorvastatin and fluvastatin on blood levels of blood-serum inflammatory cytokines and lipids in patients with unstable angina undergoing percutaneous coronary intervention.
Effect of Pseudomonas Aeruginosa on the Expressions of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in the Airway of Bronchiectasis Patients.
To investigate the association between distribution of body fat and coronary heart disease(CHD)by analysing the correlation between body fat distribution measures and incidence，type and severity of coronary heart disease.
In vitro study on relationship between posterior capsular opacification and materials of intraocular lenses.
Expression of MMP -9 and TIMP -1 in the Airway of Bronchiectasis Patients.
益气活血、泻肺利水法对慢性心力衰竭患者心功能干预的临床研究。
The D iscussion of MMP-9 and TIMP-1 in the Patients with COPD at Different Altitudes.
瓜蒌薤白半夏汤对痰浊闭阻型不稳定心绞痛患者血浆MMP-9和TIMP-1水平的影响。
THE LEVELS OF SERUM MMP-9 IN PATIENTS WITH DIFFERENT STAGES OF COPD AND COPD-PIF.
Edaravone on large cerebral infarction patients with blood serum MMP-9 expression.
Buyang Huanwu Decoction on Expression of MMP-9 in Patients with Cerebral Infarction of Qi Deficiency and Blood Stasis Type.
Prognostic Value of the Parameters of Myeloma Bone Disease Signaling in Patients with Newly Diagnosed Multiple Myeloma Treated with Proteasome Inhibitors.
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