E01M0005 Human Melatonin ELISA kit
The Human Melatonin ELISA kit can be used to identify samples from the human species. Melatonin can also be called MLT, melatonin, 5-Methoxy-N-acetyltryptamine, N-Acetyl-5-methoxytryptamine, NSC-113928.
Specifications of Human Melatonin ELISA kit
Product Information | |
Cat. No. | E01M0005 |
Product Name | Human Melatonin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MLT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MLT. Standards or samples are then added to the microtiter plate wells and MLT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MLT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MLT are added to each well to “sandwich” the MLT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MLT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLT concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Melatonin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MLT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Melatonin ELISA kit
Citations of Human Melatonin ELISA kit
E01M0005 has been referenced in the below publications：
Clinical Study on changes of plasma melatonin levels in severe hypoxic-ischemic encephalopathy neonates.
Circadian rhythm of TNF-α，IL-1β，MIF and MLT in Peripheral Blood of Ｒats with Adjuvant Arthritis.
Serum melatonin is an alternative index of Parkinson's disease severity.
