E01M0022 Human Microalbunminuria ELISA kit
The Human Microalbunminuria ELISA kit can be used to identify samples from the human species. Microalbunminuria can also be called MAU.
Specifications of Human Microalbunminuria ELISA kit
Product Information | |
Cat. No. | E01M0022 |
Product Name | Human Microalbunminuria ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MAU ELISA kit uses an anti-MAU antibody and an MAU-HRP conjugate in a competitive enzyme immunoassay method. MAU-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MAU antibody binding site between MAU from samples and MAU-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MAU. Since the number of sites is limited, as more sites are occupied by MAU from the sample, fewer sites are left to bind MAU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAU concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Microalbunminuria ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MAU and analogues was observed. | |
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Summary of the Assay Procedure for Human Microalbunminuria ELISA kit
Citations of Human Microalbunminuria ELISA kit
E01M0022 has been referenced in the below publications：
Relationship Between Klotho Gene Polymorphism and Serum Levels of Klotho Protein in Patients with Early Renal Damage in Essential Hypertension Patients.
The Relationship between In-stent Restenosis after Pereutaneous Coronary Intervention and the Concentration of Microabluminuria in the Patients with Purpose Coronary Heart Disease.
