Human Mitochondrial brown fat uncoupling protein 1 ELISA kit (E01U0090)

BlueGene Biotech Human Mitochondrial brown fat uncoupling protein 1 ELISA kit (E01U0090)

E01U0090 Human Mitochondrial brown fat uncoupling protein 1 ELISA kit

The Human Mitochondrial brown fat uncoupling protein 1 ELISA kit can be used to identify samples from the human species. Mitochondrial brown fat uncoupling protein 1 can also be called UCP1, SLC25A7, UCP, uncoupling protein 1, Thermogenin, Solute carrier family 25 member 7.

Products

Specifications of Human Mitochondrial brown fat uncoupling protein 1 ELISA kit

Product Information
Cat. No.	E01U0090
Product Name	Human Mitochondrial brown fat uncoupling protein 1 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

UCP1 ELISA kit uses an anti-UCP1 antibody and an UCP1-HRP conjugate in a competitive enzyme immunoassay method. UCP1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-UCP1 antibody binding site between UCP1 from samples and UCP1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of UCP1. Since the number of sites is limited, as more sites are occupied by UCP1 from the sample, fewer sites are left to bind UCP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UCP1 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Mitochondrial brown fat uncoupling protein 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between UCP1 and analogues was observed.